scholarly journals Rapid assessment of ceftazidime, ciprofloxacin, and gentamicin susceptibility in exponentially-growingE. coli cells by means of flow cytometry

Cytometry ◽  
1997 ◽  
Vol 27 (2) ◽  
pp. 169-178 ◽  
Author(s):  
Mette Walberg ◽  
Peter Gaustad ◽  
Harald B. Steen
Keyword(s):  
Flow Cytometry ◽  
Rapid Assessment

scholarly journals A new fluorescent probe for the equilibrative inhibitor-sensitive nucleoside transporter. 5′-S-(2-aminoethyl)-N6-(4-nitrobenzyl)-5′-thioadenosine (SAENTA)-x2-fluorescein

1991 ◽  
Vol 273 (3) ◽  
pp. 667-672 ◽  
Author(s):  
J S Wiley ◽  
A M Brocklebank ◽  
M B Snook ◽  
G P Jamieson ◽  
W H Sawyer ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Mammalian Cells ◽  
Total Concentration ◽  
Rapid Assessment ◽  
Tight Binding ◽  
Fluorescein Isothiocyanate ◽  
Molar Ratio ◽  
Nucleoside Transporter ◽  
Affinity Ligand ◽  
Leukaemic Cells

The N6-(4-nitrobenzyl) derivative of adenosine is a tight-binding inhibitor of the equilibrative inhibitor-sensitive nucleoside transporter of mammalian cells. A fluorescent ligand for this transporter has been synthesized by allowing an adenosine analogue. 5′-S-(2-aminoethyl)-N6-(4-nitrobenzyl)-5′-thioadenosine (SAENTA), to react with fluorescein isothiocyanate. The purified adduct had a SAENTA/fluorescein molar ratio of 0.92:1 calculated from its absorption spectrum. The intensity of fluorescent emission from the SAENTA-chi 2-fluorescein adduct was 30% that of fluorescein isothiocyanate (chi 2 is the number of atoms in the linkage between fluorescein and SAENTA). SAENTA-chi 2-fluorescein inhibited the influx of nucleosides into cultured leukaemic cells with an IC50 (total concentration of inhibitor producing 50% inhibition) of 40 nM. The adduct inhibited the binding of [3H]nitrobenzylthioinosine ([3H]NBMPR) with half-maximal inhibition at 50-100 nM. Mass Law analysis of the competitive-binding data suggested the presence of two classes of sites for [3H]NBMPR binding, only one of which was accessible to SAENTA-chi 2-fluorescein. Flow cytometry was used to analyse equilibrium binding of SAENTA-chi 2-fluorescein to leukaemic cells and a Kd of 6 nM was obtained. SAENTA-chi 2-fluorescein is a high-affinity ligand for the equilibrative inhibitor-sensitive nucleoside transporter which allows rapid assessment of transport capacity by flow cytometry.

Rapid assessment of the physiological status of Streptococcus macedonicus by flow cytometry and fluorescence probes

2006 ◽  
Vol 111 (3) ◽  
pp. 197-205 ◽  
Author(s):  
Konstantinos Papadimitriou ◽  
Harris Pratsinis ◽  
Gerhard Nebe-von-Caron ◽  
Dimitris Kletsas ◽  
Effie Tsakalidou
Keyword(s):  
Flow Cytometry ◽  
Rapid Assessment ◽  
Fluorescence Probes ◽  
Physiological Status

Rapid Assessment of Resection Margins During Breast Conserving Surgery Using Intraoperative Flow Cytometry

2021 ◽  
Author(s):  
George Vartholomatos ◽  
Ηaralambos Ηarissis ◽  
Maria Andreou ◽  
Vissaria Tatsi ◽  
Lamprini Pappa ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Rapid Assessment ◽  
Breast Conserving Surgery ◽  
Resection Margins

scholarly journals Rapid Assessment of the Physiological Status of the Polychlorinated Biphenyl Degrader Comamonas testosteroni TK102 by Flow Cytometry

2002 ◽  
Vol 68 (4) ◽  
pp. 2031-2035 ◽  
Author(s):  
Yoshinori Hiraoka ◽  
Kazuhide Kimbara
Keyword(s):  
Flow Cytometry ◽  
Polychlorinated Biphenyl ◽  
Rapid Assessment ◽  
Live Cells ◽  
Physiological Status ◽  
Comamonas Testosteroni ◽  
Double Staining ◽  
Permeabilized Cells ◽  
Degrading Bacterium ◽  
Double Staining Method

ABSTRACT The viability of the polychlorinated biphenyl-degrading bacterium Comamonas testosteroni TK102 was assessed by flow cytometry (FCM) with the fluorogenic ester Calcein-AM (CAM) and the nucleic acid dye propidium iodide (PI). CAM stained live cells, whereas PI stained dead cells. When double staining with CAM and PI was performed, three physiological states, i.e., live (calcein positive, PI negative), dead (calcein negative, PI positive), and permeabilized (calcein positive, PI positive), were detected. To evaluate the reliability of this double-staining method, suspensions of live and dead cells were mixed in various proportions and analyzed by FCM. The proportion of dead cells measured by FCM directly correlated with the proportion of dead cells in the sample (y = 0.9872 x + 0.18; R 2 = 0.9971). In addition, the proportion of live cells measured by FCM inversely correlated with the proportion of dead cells in the sample (y = −0.9776 x + 98.36; R 2 = 0.9962). The proportion of permeabilized cells was consistently less than 2%. These results indicate that FCM in combination with CAM and PI staining is rapid (≤1 h) and distinguishes correctly among live, dead, and permeabilized cells.

scholarly journals Rapid assessment of hyperdiploidy in plasma cell disorders using a novel multi‐parametric flow cytometry method

2019 ◽  
Vol 94 (4) ◽  
pp. 424-430 ◽  
Author(s):  
Surbhi Sidana ◽  
Dragan Jevremovic ◽  
Rhett P. Ketterling ◽  
Nidhi Tandon ◽  
Angela Dispenzieri ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Plasma Cell ◽  
Rapid Assessment ◽  
Plasma Cell Disorders
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